Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Endothelin1 induced concentrationdependent contraction of basilar arteries that was higher in diabetic than in control rabbits. Pathomorphological effects of alloxan induced acute hypoglycaemia. Alloxan diabetes alters the rabbit transarterial wall. Transarterial wall oxygen gradient measurements were performed on the infrarenal aorta with an oxygen microelectrode 14 to 16 weeks after the induction of alloxan diabetes in rabbits. Effect of alloxan time administerdrug on establishing diabetic rabbit. Histopathological abnormalities of prolonged alloxan. Neointimal thickening after catheter injury has been reported to be influenced by the integrity of the vascular endothelium.
The mechanism of dna damage induced by alloxan was investigated using 32plabeled human dna fragments. Evaluating the in vivo effects of methanolic extract of a. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. In the next step, rabbits were screened for diabetes. Evaluating the invivo effects of methanolic extract of a. Antioxidant enzymes and lipid peroxidation in alloxan. Hearts from rabbits with 816 weeks of alloxan diabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more sensitive to ischemic injury. Alloxan is known to induce diabetes in experimental animals through destruction of insulinproducing 3cells of pancreas. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Studies have shown that bone marrow adipogenesis could play an important role in the pathogenesis of diabetes 1619. After one week the survived rabbits were screened for diabetes and fasting blood glucose above 250mgdl were selected for the.
Pdf hypoglycemic effect of nyctanthes arbortristis leaf. There are a few nutritional approaches to address the increased needs of managing diabetic conditions. Rabbits were fasted for 18 h prior to alloxan administration and the dosage. Anion gap toxicity in alloxan induced type 2 diabetic rats. Phytochemical investigation of lh ii by various structural spectra showed the presence of saturated fatty acid.
The present study was undertaken with the object of determining whether the progression of cataract formation, when the latter is induced by diabetes, might be altered by the following procedures. Another 12 agematched normal rabbits were used as controls. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Alloxaninduced diabetes, a common model for evaluating the. Frontiers antihyperglycemic and antihyperlipidemic. Similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after injection of the fungus uniformly showed activation of the infection.
The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Diabetesinduced accelerated atherosclerosis in swine. For full access to this pdf, sign in to an existing account, or purchase an annual. Alloxan is a toxic glucose analogue, which selectively destroys insulinproducing cells in the pancreas that is, beta cells when administered to rodents and many other animal species. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm. In summary, with careful management, alloxaninduced diabetic rabbits can be maintained for. Hypoglycemic effect of nyctanthes arbortristis leaf extract on alloxan induced diabetic rabbits article pdf available december 2012 with 393 reads how we measure reads. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. Effect on liver enzymes due to voglibose in alloxaninduced. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Diabetic atherosclerosis was induced in rabbit iliofemoral arteries by injecting alloxan 100 mgkg, injuring the arteries using a balloon, and feeding with a 0. Garlic in alloxaninduced diabetic rabbits, the american journal of clinical nutrition, volume 28, issue 7, 1 july 1975, pages 684685, htt we use cookies to enhance your experience on our website. Cuiidependent dna damage increased with the concentration of alloxan and nadh.
However, in agreement with a previous study, a smaller group of rabbits had no evidence of diabetes despite administration of a standard dose of alloxan. Altered glucose metabolism and hypoxic response in alloxaninduced diabetic atherosclerosis in rabbits. Antioxidant enzymes and lipid peroxidation in alloxaninduced. Xanthine oxidase inhibitor allopurinol prevents oxidative. African journal of biotechnology evaluation of the. Apr 18, 2001 diabetes mellitus was induced in new zealand white rabbits using alloxan. The mechanisms of alloxan and streptozotocin induced diabetes. The basal glucose level of the animals was measured before they were induced to have type 2 diabetes. This study investigates the effect of barley hordeum vulgare, black plum syzygium cumini and chinese tree leaves pistacia chinesis aqueous extracts on serum glucose, cholesterol, urea, uric acid level and body weight of normal and alloxaninduced diabetic male rabbits. In view of the inhibitory effect on the development of experimental cholesterol atherosclerosis observed in alloxan diabetic rabbits, the effect of alloxan diabetes on the retrogression of such arterial lesions was studied in another series of experiments. Diabetes was induced in one of the groups, by intraperitonial administration of alloxan at a dosage of 150mgkg body weight and the control rabbits were administered normal saline 0. Alloxan induced diabetic rabbits as animal model for. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Increased basal phosphorylation of detrusor smooth muscle.
The mechanism of alloxan and streptozotocin action in b cells. Alloxaninduced diabetes, a common model for evaluating. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Effect on liver enzymes due to voglibose in alloxan. Boonei on liver enzyme function in alloxan induced diabetes mellitus. Effects of the specific phosphodiesterase inhibitors on. The first group 5 rabbits was used as control and fed with standard chow and tap water. The mechanisms of alloxan and streptozotocininduced diabetes. The study was carried out to evaluate the effect of alloxaninduced diabetes on some oxidative stress markers in rabbits. Pdf study of the hypoglycemic activity of hedera helix l. Postprandial antidiabetic effects of various fractions of fagonia indica burm. These protective effects were associated with reductions in ros formation and atrial fibrosis. Currently, diabetic animal models established are mainly these with.
Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. Diabetes was induced by injection of alloxan 100 mgkg body wt into the ear vein of 12wkold male new zealand white rabbits weighing. Effect of haematogenous oxidation therapy with ultravioletc irridation in alloxaninduced diabetes and poloxamer 407induced hyperlipidemia in rabbits. Effect of haematogenous oxidation therapy with ul travioletc.
Previously it has been reported that up780, a standardized composition of aloe chromone formulated with an aloe polysaccharide, has a significant impact in reducing hba1c, fasting blood glucose, fructosamine and plasma insulin level in humans and improved impaired glucose and. Pdf altered glucose metabolism and hypoxic response in. Altered glucose metabolism and hypoxic response in alloxan. Rabbits are increasingly being used as experimental diabetic models and. African journal of biotechnology evaluation of the effects. Alloxan exerts its diabetogenic action when it is administered parenterally. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. The mechanisms of alloxan and streptozotocininduced. From the 7th day of diabetes induction, blood serum was collected for glucose analysis. In normal rabbits subcutaneous granulomata produced by the injection of a spore suspension of rhizopus oryzae remained confined to the site of inoculation, showed no fungus proliferation, no longer yielded the agent on culture 10 weeks after inoculation, and eventually healed. Dec 18, 2007 alloxan has two distinct pathological effects. It has been successfully induced in a variety of animal species. Some antidiabetic properties of prosopis farcta extracts. Pdf determination of the optimal diabetes duration for.
Mechanisms underlying diabetes enhancement of endothelin. Alloxan induced diabetic rabbits as animal model for diabetes. Pdf creating a longterm diabetic rabbit model researchgate. Boonei on liver enzyme function in alloxan induced diabetes mellitus home micro biology project topics and materials evaluating the in vivo effects of methanolic extract of a. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Targeting bone marrow adipose tissue could lead to new diagnostic and therapeutic approaches for this disease.
Rabbits were kept in fasting condition for 12 h, then diabetes was induced using alloxan monohydrate 150 mgkg. An oxidation product of uric acid, 2,4,5,6pyrimidinetetrone. It is better to standardize it first by jain et al. Diabetes mellitus was induced in new zealand white rabbits using alloxan. Diabetes was induced in the rabbits with alloxan monohydrate of 150 mgdl body weight except normal and control. The effect of pregnancy complicated by alloxan diabetes on the fetuses of dogs, rabbits and rats, endocrinology, volume 40, issue 4, 1 april 1947. Rabbits having blood glucose level above 250 mgdl in fasting condition were chosen for the study as diabetes rabbits. The responses are accompanied by corresponding inverse changes in.
Similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after injection. Antihyperlipidemic effect of active principle isolated from. The mechanism of alloxan and streptozotocin action in b. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. Diabetes was confirmed after 3 days of alloxan injection by determining the fasting blood glucose concentration. Hearts from rabbits with 816 weeks of alloxandiabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more sensitive to ischemic injury. Penises excised from diabetic rabbits 8 weeks after the induction of diabetes mellitus. Alloxan, a bcell cytotoxin is used to induce chemical diabetes or alloxan diabetes by development of oxidative stress inside the cell niche, thereby damaging bcell which leads to decreased. Garlic in alloxaninduced diabetic rabbits the american. Alloxan has been administered in single or multiple.
Mar 28, 2011 in the present study, the efficacy of lh ii was evaluated for its hypolipidemic activity in alloxaninduced mildly diabetic md and severely diabetic sd rabbits. Alloxan induces damage and death of pancreatic isletcells in several experimental animal models, thus causing diabetes mellitus and decreasing the secretion of insulin 31. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. Genetic and surgical models were rarely used because they required highly technical skills and the percentage of animals lost during the procedures were higher. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxan induced diabetes. Effect of soybean extracts on serum glucose injected with alloxan monohydrate, dose 150mgkg body concentration of alloxaninduced diabetic rabbits weight. When they finish that diet, ordinary food was given to those. Effect of soybean extracts on serum glucose injected with alloxan monohydrate, dose 150mgkg body concentration of alloxan induced diabetic rabbits weight. Diabetes induced a statistically significant decrease in i p to 56% of control levels. Rabbits were weighed weekly throughout the study and the weights were recorded. In view of the inhibitory effect on the development of experimental cholesterol atherosclerosis observed in alloxandiabetic rabbits, the effect of alloxan diabetes on the retrogression of such arterial lesions was studied in another series of experiments. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxaninduced diabetes. In remaining ten rabbits, diabetes mellitus was induced by alloxan monohydrate 150 mgkg by intraperitoneal administration.
The evaluation of soybean extracts in alloxaninduced. We report herein the findings of this research work. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. Sir, we read with interest the article titled anomalies in alloxaninduced diabetic model. Research article alloxan induced diabetes mellitus in. As in humans 31,32, however, diabetic animals tended to have lower hdl cholesterol levels than nondiabetic animals, but differences in actual levels were not statistically significant, in accord with findings in alloxaninduced diabetes in swine. In our study, we found a change in the vertebral ff in alloxaninduced diabetic rabbits. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Diabetesinduced arterial wall hypoxia is proposed as an initiator and continuing stimulus for atherosclerotic vascular occlusive disease. By continuing to use our website, you are agreeing to our use of cookies. Alloxan definition of alloxan by medical dictionary. Hypoglycemic effect of aqueous extract of telfairia occidentalis leaf. Quantitative evaluation of vertebral microvascular. Antihyperlipidemic effect of active principle isolated.
Antihyperglycemic effect of persea duthieion blood glucose. Frontiers antihyperglycemic and antihyperlipidemic effects. Pdf the crude ethanolic extract of leaves of hedera helix l. A single dose of alloxan monohydrate 100 mgkg was given intravenously to 20 young new zealand white rabbits. Hypoglycemic activity of ruellia tuberosa linn acanthaceae in. Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan induced diabetic rabbit calvarial defect model. Perhaps of more relevance to the accelerated atherosclerosis in this model is that the total. Blood glucose lowering activity of aloe based composition. Effects of alloxaninduced diabetes on ischemiareperfusion.
Pdf this study was to create a longterm rabbit model of diabetes mellitus for medical studies of up. We have previously shown that neointimal thickening is significantly reduced in alloxaninduced diabetic new zealand white rabbits after catheter injury compared with. All animal studies were approved by the university of pennsylvania animal care and use committee. Research article alloxan induced diabetes mellitus in wistar. Alloxaninduced diabetes mellitus how is alloxaninduced. Our study demonstrates that allopurinol attenuated atrial structural and electrical remodeling and suppresses af vulnerability in alloxan.
The influence of alloxaninduced diabetes on the reactivity of rabbit basilar artery to endothelin1 was examined. Endothelial cell regrowth and morphology after balloon. Cataracts induced by alloxan diabetes in rats and rabbits. Treatments of the alloxaninduced diabetes rats were done by daily oral administration of different concentrations with p. In the present study, the efficacy of lh ii was evaluated for its hypolipidemic activity in alloxaninduced mildly diabetic md and severely diabetic sd rabbits. The mechanisms of alloxan and streptozotocininduced diabetes, volume 51, issue2, pp 216226 cite as the mechanisms of alloxan and streptozotocininduced diabetes alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Alloxan diabetes alters the rabbit transarterial wall oxygen.